產(chǎn)品[
Chang liver 人張氏肝細(xì)胞
]資料
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產(chǎn)品名稱:
Chang liver 人張氏肝細(xì)胞
產(chǎn)品型號(hào):
CCL-13
產(chǎn)品廠商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關(guān)文檔
簡(jiǎn)單介紹
CCL-13 Chang liver 人張氏肝細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！
Chang liver 人張氏肝細(xì)胞
的詳細(xì)介紹
CCL-13 Chang liver 人張氏肝細(xì)胞
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CCL-13?
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$323.00
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Additional information about this cell line
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HeLa [Chang Liver]
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RS Chang
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Biosafety Level:
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2 [Cells contain human papilloma virus (HPV-18) ]
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: HeLa contaminant
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keratin
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Roche FuGENE® Transfection Reagents)
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poliovirus 1, 2, 3; adenovirus 3; vesicular stomatitis (Indiana)
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Yes
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negative
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Amelogenin: X
CSF1PO: 10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
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G6PD, A
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Y
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ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
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Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
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Complete growth medium, 95%; DMSO, 5%
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22213: Chang RS. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268
26122: Murphy WH, Landau BJ. Clonal variation and interaction of cells with viruses. Natl. Cancer Inst. Monogr. 7: 249-271, 1962. PubMed: 14477443
32352: Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004
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